Ten years of next-generation sequencing technology.
- Authors
- van Dijk, Erwin L; Auger, Hélène; Jaszczyszyn, Yan; Thermes, Claude
- Year
- 2014
- Journal
- Trends in genetics : TIG
- PMID
- 25108476
- DOI
- 10.1016/j.tig.2014.07.001
Ten years ago next-generation sequencing (NGS) technologies appeared on the market. During the past decade, tremendous progress has been made in terms of speed, read length, and throughput, along with a sharp reduction in per-base cost. Together, these advances democratized NGS and paved the way for the development of a large number of novel NGS applications in basic science as well as in translational research areas such as clinical diagnostics, agrigenomics, and forensic science. Here we provide an overview of the evolution of NGS and discuss the most significant improvements in sequencing technologies and library preparation protocols. We also explore the current landscape of NGS applications and provide a perspective for future developments.
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| Title | Year | PMID |
|---|---|---|
| Statistical modeling for sensitive detection of low-frequency single nucleotide variants. | 2016 | 27556804 |
External
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| Next-generation sequencing of carbapenem-resistant Gram-negative microorganisms: a key tool for surveillance and infection control. | Bootsma HJ et al. | β | 2015 | β |
| Polymerase/DNA interactions and enzymatic activity: multi-parameter analysis with electro-switchable biosurfaces. | Langer A et al. | β | 2015 | β |
| Potential for Genetic Improvement of Sugarcane as a Source of Biomass for Biofuels. | Hoang NV et al. | β | 2015 | β |
| Reliably Detecting Clinically Important Variants Requires Both Combined Variant Calls and Optimized Filtering Strategies. | Field MA et al. | β | 2015 | β |
| Ribosome profiling reveals the what, when, where and how of protein synthesis. | Brar GA et al. | β | 2015 | β |
| RNA Amplification Protocol Leads to Biased Polymerase Chain Reaction Results Especially for Low-Copy Transcripts of Human Bone Marrow-Derived Stromal Cells. | Coenen C et al. | β | 2015 | β |
| RNA Sequencing in Schizophrenia. | Li X et al. | β | 2015 | β |
| Selection and validation of potato candidate genes for maturity corrected resistance to Phytophthora infestans based on differential expression combined with SNP association and linkage mapping. | Muktar MS et al. | β | 2015 | β |
| Sequence data for Clostridium autoethanogenum using three generations of sequencing technologies. | Utturkar SM et al. | β | 2015 | β |
| Similarity-based codes sequentially assigned to ebolavirus genomes are informative of species membership, associated outbreaks, and transmission chains. | Weisberg AJ et al. | β | 2015 | β |
| Spatially resolved transcriptomics and beyond. | Crosetto N et al. | β | 2015 | β |
| Synergisms between microbial pathogens in plant disease complexes: a growing trend. | Lamichhane JR et al. | β | 2015 | β |
| Teaching 'big data' analysis to young immunologists. | Schultze JL | β | 2015 | β |
| Teaser: Individualized benchmarking and optimization of read mapping results for NGS data. | Smolka M et al. | β | 2015 | β |
| The Haemonchus contortus kinome--a resource for fundamental molecular investigations and drug discovery. | Stroehlein AJ et al. | β | 2015 | β |
| The microbiome of the human lower airways: a next generation sequencing perspective. | Aho VTE et al. | β | 2015 | β |
| The pig gut microbial diversity: Understanding the pig gut microbial ecology through the next generation high throughput sequencing. | Kim HB et al. | β | 2015 | β |
| The search for cis-regulatory driver mutations in cancer genomes. | Poulos RC et al. | β | 2015 | β |
| The use of -omic tools in the study of disease processes in marine bivalve mollusks. | GΓ³mez-Chiarri M et al. | β | 2015 | β |
| Towards next generation CHO cell biology: Bioinformatics methods for RNA-Seq-based expression profiling. | Monger C et al. | β | 2015 | β |
| Trends in IT Innovation to Build a Next Generation Bioinformatics Solution to Manage and Analyse Biological Big Data Produced by NGS Technologies. | de Brevern AG et al. | β | 2015 | β |
| Twoβgene mutation in a single patient: Biochemical and functional analysis for a correct interpretation of exome results. | Bianco AM et al. | β | 2015 | β |
| Uncovering correlated variability in epigenomic datasets using the Karhunen-Loeve transform. | Madrigal P et al. | β | 2015 | β |
| Universal Screening for Familial Hypercholesterolemia in Children. | KlanΔar G et al. | β | 2015 | β |
| Application of next-generation sequencing technology in forensic science. | Yang Y et al. | β | 2014 | β |
| Genomic architecture of pharmacological efficacy and adverse events. | Chhibber A et al. | β | 2014 | β |
| Next-generation sequencing approach for connecting secondary metabolites to biosynthetic gene clusters in fungi. | Cacho RA et al. | β | 2014 | β |
| Profiling the microRNA Expression in Human iPS and iPS-derived Retinal Pigment Epithelium. | Wang HC et al. | β | 2014 | β |
| Roseomics: a blank slate. | Moorman NJ et al. | β | 2014 | β |
| An investigation of canine leptospiral antibodies in Tokyo and Yokohama. Comparison of Canine Positive rates between rapid microscopic agglutination test and SchΓΌffner-Mochtar test. | Ryu E et al. | β | 1974 | β |