Transcriptome analysis of frontal cortex in alcohol-preferring and nonpreferring rats.
- Authors
- Worst, Travis J; Tan, John C; Robertson, Daniel J; Freeman, Willard M; Hyytia, Petri; Kiianmaa, Kalervo; Vrana, Kent E
- Year
- 2005
- Journal
- Journal of neuroscience research
- PMID
- 15846778
- DOI
- 10.1002/jnr.20496
Although it is widely accepted that alcohol abuse and alcoholism have a significant genetic component of risk, the identities of the genes themselves remain obscure. To illuminate such potential genetic contributions, DNA macroarrays were used to probe for differences in normative cortical gene expression between rat strains genetically selected for alcohol self-administration preference, AA (Alko, alcohol) and P (Indiana, preferring), or avoidance, ANA (Alko, nonalcohol) and NP (Indiana, nonpreferring). Among 1,176 genes studied, six demonstrated confirmable, differential expression following comparison of ethanol-naive AA and ANA rats. Specifically, the mRNA level for metabotropic glutamate receptor 3 (mGluR3) was down-regulated in the AA vs. ANA lines. In contrast, calcium channel subunit alpha2delta1 (cacna2d1), vesicle-associated membrane protein 2 (VAMP2), syntaxin 1 (both syntaxin 1a and 1b; STX1a and STX1b), and syntaxin binding protein (MUNC-18) mRNAs were found to be increased in frontal cortex following comparison of AA with ANA animals. Bioinformatic analysis of these molecular targets showed that mGluR3 and cacna2d1 fall within chromosomal locations reported to be alcohol-related by the Collaborative Study on the Genetics of Alcoholism (COGA) as well as quantitative trait loci (QTL) studies. To determine further whether these differences were strain specific, the above-mentioned genes were compared in ethanol-preferring (P) and -nonpreferring (NP) selected lines. VAMP2 was the only gene that displayed statistically different mRNA levels in a comparison of P and NP rats. In conclusion, the altered cortical gene expression illuminated here would have the effect of altering neurotransmitter release in AA rats (compared with ANA rats). Such alterations, however, might not be a universal characteristic of all animal models of alcohol abuse and will also require further investigation in post-mortem human samples.
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|---|---|---|---|---|
| Metabotropic glutamate receptor 3 as a potential therapeutic target for psychiatric and neurological disorders. | Dogra S et al. | β | 2022 | β |
| Downregulation of Synaptotagmin 1 in the Prelimbic Cortex Drives Alcohol-Associated Behaviors in Rats. | Barbier E et al. | β | 2021 | β |
| Genome-wide association mapping of ethanol sensitivity in the Diversity Outbred mouse population | Parker CC et al. | β | 2021 | β |
| Loss of control over the ethanol consumption: differential transcriptional regulation in prefrontal cortex. | de Paiva Lima C et al. | β | 2017 | β |
| Voluntary wheel running reduces voluntary consumption of ethanol in mice: identification of candidate genes through striatal gene expression profiling. | Darlington TM et al. | β | 2016 | β |
| Adolescent Intermittent Alcohol Exposure: Dysregulation of Thrombospondins and Synapse Formation are Associated with Decreased Neuronal Density in the Adult Hippocampus. | Risher ML et al. | β | 2015 | β |
| DNA methylation in the medial prefrontal cortex regulates alcohol-induced behavior and plasticity. | Barbier E et al. | β | 2015 | β |
| Novel candidate genes for alcoholism--transcriptomic analysis of prefrontal medial cortex, hippocampus and nucleus accumbens of Warsaw alcohol-preferring and non-preferring rats. | Stankiewicz AM et al. | β | 2015 | β |
| Association of single-nucleotide polymorphisms in a metabotropic glutamate receptor GRM3 gene subunit to alcohol-dependent male subjects. | Xia Y et al. | β | 2014 | β |
| Eye-specific gene expression following embryonic ethanol exposure in zebrafish: roles for heat shock factor 1. | Kashyap B et al. | β | 2014 | β |
| Genetic risk prediction and neurobiological understanding of alcoholism. | Levey DF et al. | β | 2014 | β |
| Genetics and genomics of alcohol sensitivity. | Morozova TV et al. | β | 2014 | β |
| Proteomic approaches and identification of novel therapeutic targets for alcoholism. | Gorini G et al. | β | 2014 | β |
| Different alcohol exposures induce selective alterations on the expression of dynorphin and nociceptin systems related genes in rat brain. | D'Addario C et al. | β | 2013 | β |
| HSF1 transcriptional activity mediates alcohol induction of Vamp2 expression and GABA release. | Varodayan FP et al. | β | 2013 | β |
| Stress-response pathways are altered in the hippocampus of chronic alcoholics. | McClintick JN et al. | β | 2013 | β |
| Animal models for medications development targeting alcohol abuse using selectively bred rat lines: neurobiological and pharmacological validity. | Bell RL et al. | β | 2012 | β |
| Brain region-specific gene expression changes after chronic intermittent ethanol exposure and early withdrawal in C57BL/6J mice. | Melendez RI et al. | β | 2012 | β |
| Effect of metabotropic glutamate receptor 3 genotype on N-acetylaspartate levels and neurocognition in non-smoking, active alcoholics. | Xia Y et al. | β | 2012 | β |
| Gene Expression Under the Influence: Transcriptional Profiling of Ethanol in the Brain. | Contet C | β | 2012 | β |
| Alcohol induces synaptotagmin 1 expression in neurons via activation of heat shock factor 1. | Varodayan FP et al. | β | 2011 | β |
| Associations of corticosterone and testosterone with alcohol drinking in F2 populations derived from AA and ANA rat lines. | EtelΓ€lahti TJ et al. | β | 2011 | β |
| Convergent functional genomic studies of Ο-3 fatty acids in stress reactivity, bipolar disorder and alcoholism. | Le-Niculescu H et al. | β | 2011 | β |
| Molecular targets of alcohol action: Translational research for pharmacotherapy development and screening. | Gorini G et al. | β | 2011 | β |
| Candidate genes for alcohol preference identified by expression profiling in alcohol-preferring and -nonpreferring reciprocal congenic rats. | Liang T et al. | β | 2010 | β |
| Effects of acute ethanol on beta-endorphin release in the nucleus accumbens of selectively bred lines of alcohol-preferring AA and alcohol-avoiding ANA rats. | Lam MP et al. | β | 2010 | β |
| Genetic variation and brain gene expression in rodent models of alcoholism implications for medication development. | BjΓΆrk K et al. | β | 2010 | β |
| Rat genomics applied to psychiatric research. | Moisan MP et al. | β | 2010 | β |
| The complexity of alcohol drinking: studies in rodent genetic models. | Crabbe JC et al. | β | 2010 | β |
| Gene expression changes in the nucleus accumbens of alcohol-preferring rats following chronic ethanol consumption. | Bell RL et al. | β | 2009 | β |
| CNS genes implicated in relapse. | Kuntz-Melcavage KL et al. | β | 2008 | β |
| Differential gene expression in the nucleus accumbens with ethanol self-administration in inbred alcohol-preferring rats. | Rodd ZA et al. | β | 2008 | β |
| Genetic factors influencing alcohol dependence. | Mayfield RD et al. | β | 2008 | β |
| Neurogenetic networks for startle-induced locomotion in Drosophila melanogaster. | Yamamoto A et al. | β | 2008 | β |
| Functional gene expression differences between inbred alcohol-preferring and -non-preferring rats in five brain regions. | Kimpel MW et al. | β | 2007 | β |
| Identification of candidate genes for alcohol preference by expression profiling of congenic rat strains. | Carr LG et al. | β | 2007 | β |
| Identification of neurotransmitter receptor genes involved in alcohol self-administration in the rat prefrontal cortex, hippocampus and amygdala. | Pickering C et al. | β | 2007 | β |
| Phenotypic and transcriptional response to selection for alcohol sensitivity in Drosophila melanogaster. | Morozova TV et al. | β | 2007 | β |
| Integrative strategies to identify candidate genes in rodent models of human alcoholism. | Treadwell JA | β | 2006 | β |
| Longitudinal brain magnetic resonance imaging study of the alcohol-preferring rat. Part II: effects of voluntary chronic alcohol consumption. | Pfefferbaum A et al. | β | 2006 | β |
| The alcohol-preferring AA and alcohol-avoiding ANA rats: neurobiology of the regulation of alcohol drinking. | Sommer W et al. | β | 2006 | β |