Since the beginning, JASPAR has grown to provide the community with a high-quality, easy-to-use resource that promotes open science. Over the years, JASPAR’s scale and scope faithfully accompanied the technological and scientific developments in the field. Striving to ensure the high quality of the database content throughout its continuous expansion meant regularly re-visiting the sourcing, processing, and presentation of JASPAR. This effort was further oriented towards maintaining JASPAR’s ease of use, incorporating functionalities and content deliberately to address all user profiles' needs. At the age of 20, the JASPAR team looks ahead to strengthen its contribution within the open science ecosystem to which it contributed, consolidating and supporting the field in deepening our understanding of the role of TF binding in gene regulation.