Chunk #56 — Experimental Procedures — Explant Preparation and Culture

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Posttranscriptional regulation of BK channel splice variant stability by miR-9 underlies neuroadaptation to alcohol.

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Rat SON explants were prepared as previously described (Pietrzykowski et al., 2004). In transcription block experiments, water-soluble Actinomycin D (10 µg/µL, final concentration, Sigma) was added to the culture medium for the indicated time.