Chunk #57 — Experimental Procedures — Primary Striatal Cultures

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Posttranscriptional regulation of BK channel splice variant stability by miR-9 underlies neuroadaptation to alcohol.

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Cultures of dissociated rat striatal neurons were prepared using a modification of a protocol described by Leveque (Leveque et al., 2000) using postnatal day eight Sprague-Dawley rat pups. All experiments were performed on neurons that were 14–21 days in culture.