Oocytes were injected with a total of 20–50 ng of mRNA, which is less than the translational capacity of oocytes (Moar et al. 1971). We focused on the α2 and γ3 subunits as their genes are associated with alcohol related phenotypes. The β2 subunit was utilized throughout these studies as it is one of the most abundant of GABAA β subunits in the brain (Laurie, Wisden, and Seeburg 1992).
