For each experiment, all treatment and injection groups were completed and replicated in at least 3 individual batches of oocytes from different donors to confirm the reproducibility of the findings. Peak current amplitudes were measured in individual oocytes after GABA treatment and averaged within treatment and injection groups. Data are displayed as the average ± SEM in bar graphs with the number of oocytes tested indicated in the results and/or figure legend. For GABA concentration response experiments, peak current amplitudes for each GABA concentration were normalized to the peak current amplitude from the 30 μM GABA response for each oocyte and averaged within groups. Non-linear regression analysis was used to fit the data and determine GABA EC50 values. For acute ethanol treatment experiments where oocytes served as their own controls the data were normalized to peak current amplitude before ethanol and averaged within groups. In chronic ethanol treatment experiments, individual GABA currents were normalized to the average current amplitude of control groups with no ethanol treatment within the same experiment and the mean ratios ± SEM are displayed in bar graphs. Paired t-tests or ANOVA with Tukey’s posthoc analysis was used to compare groups.
