Ingenuity® pathways analysis indicated several networks that were altered by ethanol drinking (Table 3). In 4 of the 5 networks, more genes had higher than lower expression levels in the ethanol compared to the water group, with the transcription and glucocorticoid receptor signaling networks having the highest ratios. Although several networks fell within the same general categories in both the CeA and ACB-shell (i.e., transcription, long-term potentiation, and glucocorticoid receptor signaling), there was almost no overlap between the 2 regions in the genes that changed within each category. In addition, networks involved in axonal guidance signaling and ephrin receptor signaling were altered in the CeA, but not ACB-shell, whereas Wnt/beta-catenin signaling and NFKB signaling were altered in the ACB-shell, but not the CeA, of the ethanol group (Table 3).
