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Chunk #30 — Methods — Microfluidic qPCR (single cell samples)

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Neurotoxic reactive astrocytes are induced by activated microglia.
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Experiments were performed on mice from the transgenic mouse line Tg(Aldh1l1-EGFP)OFC789Gsat/Mmucd. For neuroinflammatory injury, postnatal day 5 (P5) mice received a single intraperitoneal injection of either endotoxin-free PBS, or the endotoxin lipopolysaccharide (LPS) from E. coli O55:B55 (Sigma-Aldrich) dissolved in normal saline and diluted into endotoxin-free PBS (5 mg/kg).