cDNA samples from the stimulation experiments were measured together with no reverse transcriptase and no template controls on 96.96 Dynamic Array chips (Fluidigm). Cell-type specific transcripts were also detected for microglia, oligodendrocyte lineage cells, and neurons, with any astrocyte samples containing measurable levels of other cell types removed from further analysis. All primer sequences for rat and mouse are listed in Supplemental Data Tables 1 and 2.
