Figure 1 shows representative anatomical evidence for eNpHR3.0 expression in the PL (panel A’), eNpHR3.0 expression in NAcore neurons (panel B’) and ArchT expression in PL fibers terminating in the NAcore (panel C’). Whole cell patch clamp of a pyramidal neuron in the PL shows that optically inhibiting the infected region hyperpolarizes neurons and decreases action potential generation (Fig. 1a’’). Similarly, optically inhibiting NAcore medium spiny neurons reduced membrane potential and inhibited neuron firing (Fig. 1b’’). Figure 1c shows the results of optically inhibiting field potential amplitude in the NAcore that was elicited by electrically stimulating the PL. eNpHR3.0 was administered in the PL, and an optical fiber and recording electrode were inserted into the NAcore. Increasing laser intensity produced a progressive reduction in field amplitude. The capacity of activating eNpHR3.0 to reduce field potentials endured for at least 2 hours.
