Rats were transcardially perfused with 4% paraformaldehyde. Coronal sections were evaluated for yellow fluorescent protein (YFP) either by immunocytochemistry or using a helium/neon 543 nm laser line with a YFP excitation filter. Because a Kolmogorov–Smirnov test revealed that some of the reinstatement data were not normally distributed, a Friedman repeated measures nonparametric test was employed followed by a Dunn’s multiple comparison. The locomotor data were evaluated using a two-way analysis of variance with repeated measures over time, followed by a Bonferroni multiple comparisons test.
