Chunk #59 — PROCEDURE — hESC (HUES 9) culture and transfection ● TIMING 3–4 d

Source

Genome engineering using the CRISPR-Cas9 system.

Embedded

yes

Text

▲ CRITICAL STEP Do not dissociate the cells mechanically without Accutase. 52Spin the cells down at 200g for 5 min at room temperature.