Chunk #60 — PROCEDURE — hESC (HUES 9) culture and transfection ● TIMING 3–4 d

Source

Genome engineering using the CRISPR-Cas9 system.

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yes

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▲ CRITICAL STEP Do not spin the cells without inactivating the Accutase first, or above the recommended speed; doing so may cause cells to lyse. 53Process pelleted cells directly for DNA extraction with the QuickExtract solution (Steps 71–74).