Similar results were obtained in female mice. Preference for ethanol was significantly reduced in five of six mutant strains of female mice as shown in Fig. 2 [main effect of genotype: F(1,96) = 9.2, p<0.01 for B2m mice; F(1,85) = 47, p<0.001 for Ctss mice; F(1,63) = 130, p<0.001 for Il1rn mice; F(1,80) = 40, p<0.001 for Cd14 mice; F(1,90) = 20, p<0.001 for Il6 mice]. The amount of ethanol consumed was also significantly reduced in these same five strains of knockout female mice. However, no differences in preference for ethanol or amount of ethanol consumed were found between wild type and Ctsf female mice. Total fluid intake was reduced in B2m and Ctss null female mice (as it was for the male mice in these two strains) and increased in Cd14 and Il6 knockout female mice (for complete results see Supplemental Fig. 3 and Fig. 4; for detailed statistics see Supplemental Table 2).
