In a two-bottle free-choice paradigm in which mice could drink either water or a series of increasing ethanol concentrations, the preference for ethanol was significantly reduced in all six mutant strains of male mice as shown in Fig. 1 [main effect of genotype: F(1,80) = 7.4, p<0.01 for B2m mice; F(1,90) = 81, p<0.001 for Ctss mice; F(1,64) = 5.2, p<0.05 for Ctsf mice; F(1,58) = 15, p<0.001 for Il1rn mice; F(1,90) = 19, p<0.001 for Cd14 mice; F(1,90) = 41, p<0.001 for Il6 mice]. The amount of ethanol consumed was also significantly reduced in B2m, Ctss, Ctsf, Il1rn and Il6 knockout male mice. Total fluid intake was reduced in B2m and Ctss null male mice and increased in Cd14 knockout male mice (for complete results see Supplemental Fig. 1 and Fig. 2; for detailed statistics see Supplemental Table 1).
