iPSC lines were characterized for expression of stem cell markers by RT-PCR and immunofluorescence microscopy as previously described (Trokovic et al., 2013, Trokovic et al., 2014). For immunofluorescence analyses we used stem cell-specific antibodies against TRA-1-60 (Millipore, MAB4360), NANOG (Cell Signaling, D73G4), SSEA4 (Millipore, MAB4304), and OCT4 (Santa Cruz Biotechnology, sc-9081). Cell nuclei were stained with DAPI (Vectashield, Vector).
