Fibroblasts were grown from skin biopsies under glass plates in DMEM + 20% fetal calf serum and antibiotics. Mononuclear cells were extracted freshly from blood by Ficoll-extraction method. PBMCs (1 × 105 to 1 × 106) and fibroblasts were transduced with SeVdp as previously described (Nishimura et al., 2011, Trokovic et al., 2014). Cells were plated on mitomycin C-treated murine embryonic fibroblasts (3.75 × 105 feeder cells/well) on a six-well plate in hES medium: DMEM/F12 with GlutaMAX, supplemented with 20% KO-serum replacement, 0.1 mM β-mercaptoethanol, 1% non-essential amino acids (all from Life Technologies), and 6 ng/ml basic fibroblast growth factor (bFGF; Sigma). For feeder-free cultures iPSC lines were grown on Matrigel (growth factor reduced, BD Biosciences) in StemPro (Life Technologies) or in Essential-8 medium (E8, Life Technologies). The donor identity of all iPSC lines was confirmed by microsatellite marker analyses.
