Quantitative PCR was carried out on reverse-transcribed RNA from fly heads according to the manufacturer’s instructions (Applied Biosystems) on an ABI PRISM 7900 Sequence Detection System, using expression levels of RpL32 as a standard to normalize sample concentrations. TaqMan probesets (Applied Biosystems) used in this study were dally: Dm01822385_g1, dlp: Dm01798599_g1, and RpL32: Dm02151827_g1.
