Sleep/wake analysis. For the entire period of monitoring, each mouse was placed in its own chamber of a Piezo-electric grid and chamber system (Donohue et al. 2008). The Piezo chamber detects movement, and software analysis of respiration rates determine sleep or wake for each mouse. The mice had access to food and water ad lib while in the chamber. The room was maintained on a 12:12 light:dark cycle. Mice were placed in the chambers between 0900 and 1000 h on Day 1 and were removed on Day 5 at the same time. Each day, the computer, food and water were checked. Otherwise, the mice remained undisturbed.
