Porsolt forced swim test. Mice were tested in the Porsolt forced swim test at least 72 h after completion of the sleep analysis. The water in the chamber was heated to 25°C. Each mouse was placed in the chamber for 5 min and videotaped. The water was changed for every third mouse. The behavior of each mouse was scored from the videotapes. The total time immobile as well as the time immobile for the last 3 min were scored and analyzed.
