Sequences of all plasmids used in this study are provided in Appendix S1, and all cloning procedures and targeting experiments are described in detail in the supplementary Materials and Methods. Briefly, AAVS1 targeting for OPTiKD and sOPTiKD was performed by lipofection, while AAVS1 targeting for sOPTiKO was performed by nucleofection (Bertero et al., 2015; Vallier et al., 2004). Clonal lines were selected using 1 μg/ml puromycin (Sigma; for OPTiKD and sOPTiKD) or 25 μg/ml geneticin (G418 sulfate, Gibco) and 0.5 μg/ml puromycin (for sOPTiKO).
