Mice were maintained on a 12 h dark/light cycle and fed a normal chow diet. Unless otherwise indicated, all mice were 14–16 weeks old at the time of experiments. Mice were bred in-house in the University of Texas Southwestern Medical Center and Lilly animal facilities. The HFD consisted of 60 % (kCal) from fat (Research Diets, D12492). Lepob/ob were on an FVB background to allow for uniform development of hyperglycemia; all other mice were on C57Bl6/J backgrounds. The Institutional Animal Care and Use Committee of the University of Texas Southwestern Medical Center and Lilly and Company approved all animal experimental protocols.
