The 293T human embryonic cell line was originally obtained from ATCC (CRL-3216). The HAP1 Human Male Chronic Myelogenous Leukemia (CML) cell line was obtained from Horizon Discovery Life Sciences. Human lymphoblastoid cell lines were generated by EBV immortalization of primary human lymphocytes obtained from patient blood samples. 293T human embryonic kidney cell lines were cultured in Dulbecco’s Minimal Essential Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1X penicillin and streptomycin (ThermoFisher), and 1X Glutamax (Gibco) at 37 °C with 5% CO2. Cells were passaged every 3 days with 0.25% Trypsin. Human HAP1 DALRD3 knock-out cell lines were generated by CRISPR/Cas9 mutagenesis (Horizons Discovery Life Sciences). Human HAP1 cell lines were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) supplemented with 10% FBS and 1X penicillin and streptomycin at 37 °C with 5% CO2. Cells were passaged with 0.05% Trypsin. Human lymphoblastoid cell lines were cultured in Roswell Park Memorial Institute (RPMI) 1640 Medium containing 15% fetal bovine serum, 2 mM l-alanyl-l-glutamine (GlutaMax, Gibco) and 1% Penicillin/Streptomycin.
