We reverse-transcribed 1 μg of total RNA with oligo-dT primers using the Transcriptor First Strand cDNA Synthesis kit according to the manufacturer's instructions (Roche Applied Science, Indianapolis, Indiana). Next, we amplified a total of 5 ng of reverse-transcribed RNA in triplicate reactions with 1 assay for SLC1A1 and 1 endogenous control assay for PGK1. The SLC1A1 assay was designed with the Universal Probe Library Assay Design Center, version 2.4 (Roche Applied Science) using primers GCT GTC GAC TGG CTC CTG and GGA GAG CTT TTC CAC AAT GC (Operon Biotechnologies, Huntsville, Alabama) and Universal Probe Library probe No. 9 (Roche Applied Science). The PGK1 assay was predesigned and purchased directly from the vendor (Universal Probe Library Human PGK1 Gene Assay, Roche Applied Science). Both assays span introns and do not contain known SNPs in primers or probes. Amplification was carried out in a 10-μL total reaction volume with 500 nM of each primer and 100 nM of fluorescent probe using PerfeCta qPCR FastMix (Quanta BioSciences, Gaithersburg, Maryland). Thermocycling consisted of 2 minutes at 45°C for uracil-N-glycosylase incubation, 30 seconds at
