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Chunk #2 — METHODS — REAL-TIME REVERSE TRANSCRIPTASE–POLYMERASE CHAIN REACTION QUANTITATION OF SLC1A1 IN POSTMORTEM BRAIN TISSUE

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A haplotype containing quantitative trait loci for SLC1A1 gene expression and its association with obsessive-compulsive disorder.
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We obtained RNA and genomic DNA from 105 postmortem dorsolateral prefrontal cortex samples through the Stanley Medical Research Institute, Chevy Chase, Maryland. This collection is composed of 3 diagnostic groups (35 normal, 35 bipolar, and 35 schizophrenic donors). With the exception of 2, all samples were from white donors. Detailed demographics of this sample are available at http://www.stanleyresearch.org. Six of these 105 samples did not provide sufficient RNA for quantitation, and the 2 non-white samples were quantitated but not analyzed, resulting in a total of 97 samples, which are presented herein. All samples were successfully genotyped for rs3933331, rs7858819, and rs301430.