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Chunk #10 — Methods — Analysis of microglial immunofluorescence

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Chronic alcohol-induced neuroinflammation involves CCR2/5-dependent peripheral macrophage infiltration and microglia alterations.
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Microglia were identified within the hippocampus, and soma area and the perimeter length of microglial extensions were measured using Fiji v1.0. Somas were measured by drawing a circumferential outline along the edge of the cell body to the base of any cellular extensions. The perimeter length was measured by summing the lengths of all cellular extensions.