Brain tissue was dissected from mice perfused with PBS/heparin, and the hippocampus, cerebellum, and cortex tissue were stored in RNA later. The cortex tissue was defined as the superficial tissue (from the surface to approximately 0.5 mm deep) dissected approximately 2 mm anterior, 2 mm posterior, and 3 mm lateral with respect to the bregma, a region that encompasses the motor and somatosensory cortex. RNA was extracted using the miRNeasy Kit (Qiagen, Germantown, MD, USA) with on-column DNAse digestion (Zymo Research, Irvine, CA). Concentration was determined using a Nanodrop 2000 (Thermo Scientific, Waltham, MA, USA), and 1 μg RNA was used for cDNA reverse transcription (BioRad, Hercules, CA, USA). Quantitative real-time polymerase chain reaction (qPCR) was completed using SYBR Green polymerase (BioRad) and expression measured on a BioRad CFX96 Real Time System. qPCR primers are listed in Table 2, and the expression was quantified by using the 2−ΔΔCt method. Table 2Real-time PCR primersForwardReverseNanostring hits C1qaCAAGGACTGAAGGGCGTGAAGGGGCTGGTCCCTGATATTG C1qbGGTGCCAACAGCATCTTCACTTTGACCCCGTGATTACGCA DaxxCTATAGGCCAGGCGTTGACCGTTCGATTTTCCCGAAGGCG GnasATGGGTTTAACGGAGAGGGCACCATCGCTGTTGCTCCTTG Hmgn1CTCCTCGGTGACAGATCCGAAACCTTCCTCTTGGGCATCG Hspb1CTGGCAAGCACGAAGAAAGGGCACCGAGAGATGTAGCCAT Il-23TGGTTGTGACCCACAAGGACCAGACCTTGGCGGATCCTTT Map3k9TGGGCAGAAAGAGCTCACATACATCATCTGCCTCTTACCCTTC Mapk1CAGTTTGTCCCCTTCCATTGATACTCCCACAATGCACACGAC Mef2aAGCACTTTGAAAGGAAGAGTCCAAGCTCCCCCACTGCACATTA Myd88AGGCATCACCACCCTTGATGCGAAAAGTTCCGGCGTTTGT Plcb1AGATCCTCGATGAGAAGCCCCTTCCGACAAGACTGAGGAGG Tgfβ1GTCACTGGAGTTGTACGGCAAGCCCTGTATTCCGTCTCCT Itgb2TTCCTGGTGCCAGAAGCTGAACCCCGTTGGTCGAACTCAG Ccl11TGCAGGCAGTTTTCTCTGGAAGGCTCTCCCGACTAGCTTTProinflammatory TnfαGAAGTTCCCAAATGGCCTCCGTGAGGGTCTGGGCCATAGA Tlr4TCAGAACTTCAGTGGCTGGAAGAGGTGGTGTAAGCCATGC Cox2AACCGAGTCGTTCTGCCAATCTAGGGAGGGGACTGCTCAT Ym1CAGAAGCTCTCCAGAAGCAATTGCCAGACCTGTGACAAGAAT Il-1βTCTTTGAAGTTGACGGACCCTGAGTGATACTGCCTGCCTG Il-17CAGGGAGAGCTTCATCTGTGTGCTGAGCTTTGAGGGATGATCCR2/5 network Ccl2CCACAACCACCTCAAGCACTAGGCATCACAGTCCGAGTCA Ccl3ATATGGAGCTGACACCCCGATCAACGATGAATTGGCGTGG Ccr2GTGTACATAGCAACAAGCCTCAAAGCCCCCACATAGGGATCATGA Ccr5TGGGGTGGAGGAGCAGGGAGTAGGCCACAGCATCGGCCCTHousekeeping GapdhGGCAAATTCAACGGCACAGTGATGGGCTTCCCGTTGATGA 18S rRNAGTAACCCGTTGAACCCCATTCCATCCAATCGGTAGTAGCG
