Chunk #12 — Methods — Nanostring gene expression analysis

Source

Chronic alcohol-induced neuroinflammation involves CCR2/5-dependent peripheral macrophage infiltration and microglia alterations.

Embedded

yes

Text

RNA was extracted as above. RNA and Nanostring reactions were prepared according to the manufacturer’s recommendation for the Mouse nCounter Inflammation Panel (Nanostring Technologies, Seattle, WA, USA). Data were analyzed using the nSolver Analysis Software 3.0 (Nanostring Technologies).