Chunk #61 — Methods — RNA-sequencing — Pre-processing of sequence data

Source

Common genetic variation drives molecular heterogeneity in human iPSCs.

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yes

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Raw RNA-seq reads were aligned using STAR v2.4.0 63 against the 1000 Genomes Phase2 reference genome assembly that integrates the GRCh37 primary assembly with the human decoy sequence 37d5. Exon-intron junctions derived from Gencode v19 transcript annotations 56 were used to improve the alignments.