Statistical analyses were conducted using Statview or JMP 7 (SAS Institute). Data are presented as mean ± s.e.m. For DNA methylation analysis, the percentage of methylated clones for each subject was tabulated by dividing the number of clones with at least one methylated CpG site by the total number of clones. A factorial ANOVA was used to compare the percentage of methylated clones for each subject as the dependent variable and group (suicide abused, suicide nonabused or control) as the between groups factor. To examine differential methylation across CpG sites methylated in vitro, we compared the total number of methylated CpG sites across the NR3C1 promoter (n = 33) for all clones per group (that is, 12 subjects × 20 clones = 240 clones per group) using ANOVA followed by Bonferroni corrected post hoc comparisons. For RNA expression analysis, ANOVA followed by PLSD post hoc tests were used to examine differences between the suicide victim and control group. Unpaired t tests were used to compare groups of subjects with different clinical diagnoses (for example, subjects with mood disorders versus subjects
