is the highest fluorescence intensity value following KCl addition. To investigate signal change after CNO/clozapine addition, two 90 second windows, equivalent to 818 frames, on either side of the addition were considered. The first 90 second window lasted from frame 80 to 898. The second window after the addition of CNO/clozapine lasted from frame 1580 to 2398, which are the latest time points of the pre- and post- CNO addition timelines. Ten cells from both the excitatory and inhibitory sides were then individually analyzed.
