To quantify network activity, raw TIFF images of GCaMP6f fluorescence were collected at 9 Hz (frames per second) using the IN Cell Analyzer with a GFP bandpass filter. Using image processing software FIJI, images were stacked, concatenated, and uniformly contrast adjusted (to 85 and 535 intensity values). AVI videos were saved at 70 Hz. For region of interest (ROI) analysis, ROIs were drawn and stored in ImageJ’s ROI manager. The Multi Measure function in ImageJ was used to collect signal intensity information. To process the data as a ratio of signal over baseline, a custom MATLAB script which collects the lowest fluorescence intensity value from the first 200 frames, represented as F0, was used. Fluorescence signals were then normalized using the ratio calculation (F – F0)/(Fsat – F0), where F is the measured fluorescence signal within an ROI and Fsat is the highest fluorescence intensity value following KCl addition. To investigate signal change after CNO/clozapine addition, two 90 second windows, equivalent to 818 frames, on either side of the addition were considered. The first 90 second window lasted from frame
