To examine the effect of acute alcohol exposure on GABAA-receptor-mediated chloride currents in neurons from CTLs and ADs, a micropipette attached to a positive-pressure applying Picospritzer (Parker Hannifin Corp) was filled with aCSF containing 50 μM GABA and placed ≈20–30 μm away from the patched neuron. GABAA-mediated currents were examined in voltage clamp mode with the cell held at −70 mV. Currents were evoked every 30 sec by a 50–200 mS focal GABA application at 5–10 PSI. Following a 5-min baseline recording in normal aCSF, the perfusion was switched to an aCSF containing 50 mM alcohol for 15 minutes, following which a normal aCSF was perfused for a 15-min washout period.
