Chunk #43 — STAR Methods — Experimental model and subject details — Human ESC Differentiation

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Single-Cell Profiling of an In Vitro Model of Human Interneuron Development Reveals Temporal Dynamics of Cell Type Production and Maturation.

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For RNAseq analysis, samples were taken from at least 3 separate experiments that passed our quality control metrics. Our quality control metrics included: 1) a minimum percentage (70%) of FOXG1 and NKX2-1 positive cells by immunofluorescence at D24; 2) a minimum percentage (10%) of Citrine+ cells by FACs analysis at D24; 3) a minimum (30%) percentage of SST+ cells by immunofluorescence at D54.