Chunk #42 — STAR Methods — Experimental model and subject details — Human ESC Differentiation

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Single-Cell Profiling of an In Vitro Model of Human Interneuron Development Reveals Temporal Dynamics of Cell Type Production and Maturation.

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On D25, medium was replaced with NBND medium for neuronal differentiation (Neurobasal medium, 0.5× N-2 supplement, 0.5× B-27 supplement, 2 mM Glutamax supplement, 0.025% Bovine Albumin Fraction V Solution, 1X Penicillin-Streptomycin, 55 μM 2-Mercaptoethanol, 0.2 mM Sodium-L-Ascorbate (Sigma Aldrich), 0.1 mM Adenosine 3′,5′-cyclic monophosphate (Sigma Aldrich), 5 ng/mL NT-3 (R&D Systems), 5 ng/mL BDNF (R&D Systems), 5 ng/mL GDNF (R&D Systems). Half the medium was replaced every 48 h for the duration of the experiment.