For the WGCNA, only by using a very liberal p-value of 0.10 as the cutoff for gene inclusion (2193 genes in the Acb-shell; 2395 genes in the CeA) did the resultant networks meet the criteria for scale free topology (Zhang & Horvarth, 2005). Modular colors indicate clusters of highly interconnected genes and are randomly assigned by the program. In the Acb-sh, the Blue module (neuronal enrichment) contained 21 significant GO categories with 40 or more genes; in the CeA, the Yellow module contained one category and the Turquoise module (astrocytes and oligodendrocytes enrichment) contained 19 GO categories with 40 or more genes (Table 5). There were no categories that were similar between the Acb-sh and CeA. However, there were two signaling pathways that could alter neuronal function in each region, the G-protein coupled receptor signaling pathway in the Acb-sh and the transmembrane receptor protein tyrosine kinase signal pathway in the CeA.
