Sufficient RNA was available in the Acb-sh samples to validate a few key genes using qRT-PCR. Five genes were selected (Table 6); these genes were significantly different in both the Acb-sh and CeA between the alcohol drinking and water control groups and had a fold-change of approximately 1.4 or higher in the Acb-sh. There was excellent agreement between the qRT-PCR values and the values obtained with the microarray procedure (Table 6).
