Three weeks after viral stereotactic injections, AAV-CLIC4 (Clic4 over-expressing) and AAV-IRES (control) injected mice were evaluated for a sequential battery of behavioral responses to acute ethanol administration. Mice were habituated to injections with saline in their home cage for 2 days prior to behavioral studies. All animals were allowed a 1-hour acclimation period to the behavioral room prior to testing. Locomotor activity was measured immediately following injection with either saline or ethanol (2.0 g/kg) by photobeam breaks during a 10-minute session in locomotor activity chambers (Med-Associates, model ENV-510; St. Albans, VT) in ventilated sound attenuating boxes (ENV-022MD-27). Two weeks later, anxiety testing was done using the light-dark transition model (Crawley & Goodwin, 1980). Mice were injected i.p. with saline or ethanol (1.8 g/kg) and placed in the light compartment of the light-dark box (Med-Associates ENV-510 with black plexiglass inserts to divide into two 13.5 cm × 27 cm × 20 cm compartments. Light compartment was illuminated with 100 mA incandescent bulbs. Timing was initiated when animals entered the dark compartment and locomotor activity/position monitored for 10 minutes. Results were expressed
