Helper-free adeno-associated serotype 2 was used throughout this study. Mouse Clic4 cDNA was generated by PCR and cloned into the plasmid pAAV-IRES-hrGFP (Stratagene, La Jolla, CA) in frame with the FLAG epitope tag. FLAG-tagged pAAV-CLIC4-GFP was verified through sequencing and by green fluorescent protein (GFP) expression in HEK-293 cells via Arrest-in transfection (Open Biosystems, Huntsville, AL). Additional details are provided as Supplementary Material.
