Total liver RNA was isolated using RNeasy Mini kits, according to manufacturer’s instructions (Qiagen, Valencia, CA). RNA concentration was quantified and the purity determined by A260/A280 ratio and by visual inspection via denaturing gel electrophoresis. All Agilent mouse microarray (Santa Clara, CA) analyses were performed with three biological replicates from WT and Cyp1b1-ko mice fed either the LFD or HFD. All methods used for microarray preparation were completed as previously described [28]. All arrays were normalized and quality assessed using EDGE software. ANOVA analysis was completed to determine statistical significance.
