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Chunk #5 — Materials and methods — Animals

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Chronic ethanol increases systemic TLR3 agonist-induced neuroinflammation and neurodegeneration.
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Eight-week-old male (20 to 22 g) C57BL/6 mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA). All protocols and procedures in this study were approved by the Institutional Animal Care and Use Committee (IACUC) and were in accordance with the National Institute of Health regulations for the care and use of animals in research.