Poly I:C was purchased from Amersham Biosciences (Piscataway, NJ, USA). Goat polyclonal TLR3 (N-14) and HMGB1 (K-12) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Rabbit anti-Iba1 antibody was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Monoclonal anti-mouse gp91phox was from Transduction Laboratories (Lexington, KY, USA). Rabbit polyclonal MAP-2 antibody was purchased from Abcam (Cambridge, MA, USA). Polyclonal rabbit anti-glial fibrillary acidic protein antibody was from DakoCytomation (Glostrup, Denmark). Cleaved caspase-3 (Asp175) antibody was from Cell Signaling Technology (Danvers, MA, USA). Fluoro-Jade B and mouse NeuN antibody were from Chemicon International (Temecula, CA, USA). TNFα, IL-1β, MCP-1 and IL-6 ELISA kits were purchased from R&D Systems Inc. (Minneapolis, MN, USA). Hydroethidine was from Invitrogen Molecular Probes (Eugene, OR, USA). All other reagents came from Sigma-Aldrich Chemical Company (St. Louis, MO, USA).
