Time course of TNFα response to TLR3 agonist poly I:C: Male C57BL/6 mice were intraperitoneally (i.p.) injected with a single dose of poly I:C (250 μg/kg) and d-(+) galactosamine hydrochloride (d-GalN 20 mg/kg), or saline (control). Mice were sacrificed at selected time points, and sera and brain samples were used for TNFα measurement by ELISA.
