is a significant component of ischemic stroke. For examining the influence of Wy-14643 and fenofibrate in non-LPS injected brain, 5 animals were analyzed in each treatment group. Ten LPS-injected animals for each treatment were randomly subdivided into two groups with a survival period of 6 hours or 3 days after LPS. In the 3 days survival group, the treatment with PPARα agonists was continued for 3 days after LPS. In each LPS-injected animal, the injection was made on both sides at 1 hour after the seventh drug administration. Additional 10 animals that received intracerebral injection with saline but without PPARα agonist treatment served as control (n = 5 for both 6 hours and 3 days). To test the effect of post-LPS treatment with fenofibrate, vehicle or fenofibrate (30 mg/kg) was given 15 minutes after LPS injection, followed by daily administration until brain was collected at 3 days after LPS injection (n = 6 and 7 for vehicle and fenofibrate, respectively).
