GABA concentration response curves were determined to compare GABA current characteristics in cells expressing differing subunit ratios (Figure 2B and 2C). EC50 values for the GABA response did not differ between oocytes expressing 2:2:1 (95% confidence interval = 1.65–2.93 μM, n = 9) and 6:2:1 (95% confidence interval = 1.37–3.36 μM, n = 12) ratios of subunits. The GABA concentration of 30 μM was saturating; 100 μM GABA elicited responses equal to or less than the 30 μM concentration (data not shown).
