To more accurately reflect the most common native structure of GABAA receptors (two α, two β and one γ subunit), we examined the effects of increasing or decreasing the relative abundance of the α2 subunit compared to a baseline ratio of 2:2:1. As seen in Figure 2A, the 2:2:1 ratio group (316 ± 51 nA, n = 52) produced significantly greater currents in response to 10 μM GABA than the 6:2:1 ratio group (133 ± 19 nA, n = 36). In these experiments, the 0.5:2:1 group (304 ± 66 nA, n = 24) did not significantly differ from the baseline 2:2:1 group.
