Shiverer x rag2-null mice were sacrificed at 4.5 months of age and their white matter astrocyte morphologies assessed. 150 μm thick coronal slices were taken by Vibratome at Bregma -1.0 mm from control (22, 37 and C27) or SCZ (51, 164, 193) hGPC-engrafted mice, incubated in mouse anti-hGFAP for 1 week, then 4 hrs in Alexa 568 goat anti-mouse. The slices were mounted on slides and imaged at 100× by confocal (Leica SP8). The images were traced using Neurolucida 360 (MicroBrightfield, Inc.); all tracings were done by experimenters blinded as to the treatment condition.
