Individual astrocytes were selected from the middle of the corpus callosum at mid-depth so as to capture cells and their processes in their entirety. Nine cells/brain were analyzed by Neurolucida with Sholl analysis, as 3 cells/slice and 3 slices/brain, taken at 500, 1000, and 1500 μm lateral of the midline. Two or three brains were assessed for each of three lines produced from separate patients, for a total of 8 brains and 72 traced cells/condition, for both CTRL and SCZ-engrafted groups. For Sholl analysis, concentric shells placed at successively increasing diameters of 5 μm were centered on the cell body, and the number of intersections between cell processes and shells counted (Sholl, 1953). For the assessment and quantitative description of astrocytic fiber 3D architecture, Fan-in analysis (MBF Biosciences) was used as previously described for studies of dendritic topology (Dang et al., 2014).
