To measure forebrain myelination, we used luminance analysis based on measurement of myelin basic protein (MBP) immunofluorescence. Evenly-spaced and uniformly sampled coronal sections were stained for MBP as described, and images taken at 10× using a Nikon Ni-E and Nikon DS-Fi1 camera. The corpus callosum was selected as region of interest, and mean intensity values were obtained using NIS Elements v.4.5.
